Medical School
Twin Cities
This study aims to use bioinformatics tools to construct a comprehensive gene regulatory network centered around CPEB4 in both human cardiac muscle cells and mouse heart tissue. By analyzing this network, the researchers seek to uncover the molecular mechanisms through which CPEB4 influences cardiac ion channel regulation, potentially leading to the development of predictive markers for arrhythmia risk and CPEB4-based interventions for preventive and therapeutic approaches. RNA samples will be obtained from hiPSC-CMs of control and CPEB4 KD groups, and from cardiac-specific Cpeb4 cKD mouse heart tissue. Non-transfection or transfection reagent only controls in iPSC-CMs can be added to investigate off-target effects of control shRNAs. Additionally, tamoxifen-treated Myh6-cre/Esr1; Cpeb4+/+ controls can be added to investigate the effect of tamoxifen-induced cardiac toxicity in Myh6-cre/Esr1 Tg mice.